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fibroblast growth factor 2  (R&D Systems)


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    Structured Review

    R&D Systems fibroblast growth factor 2
    Fibroblast Growth Factor 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 908 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+basic+fgf/Recombinant+Human+FGF+basic%2FFGF2%2FbFGF+(146+aa)+Protein/pm42822105-87-45-50
    Average 96 stars, based on 908 article reviews
    fibroblast growth factor 2 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Generated:

    Article Title: MYC CONTROLS HUMAN PLURIPOTENT STEM CELL FATE DECISIONS THROUGH REGULATION OF METABOLIC FLUX
    Article Snippet: .. Pre-neural crest stem cells (NCSC) were generated by culturing hESCs or hiPSCs at 90,000 cells/cm 2 on Geltrex, diluted 1:200 with DMEM/F-12, coated plates for 6 days in DM supplemented with 10 ng/mL human Heregulinβ-1, 200 ng/mL LONG® R3 human IGF-I, 8 ng/mL human basic-FGF, 20 μM SB431542 and 0.2 μM BIO (R&D Systems, 3194/10). ..

    Article Title: Human beige adipocytes for drug discovery and cell therapy in metabolic diseases
    Article Snippet: DM was comprised of DMEM/F-12 w/o glutamine supplemented with 2% Probumin Bovine Serum Albumin (EMD Milipore, 821005), 1× Antibiotic-Antimyotic (Corning, 30-004-CI), 1× MEM non-essential amino acids (Corning, 25-025-CI), 1× Trace Elements A (Corning, 99-182-CI), 1× Trace Elements B (Corning, 99-175-CI), 1× Trace Elements C (Corning, 99-176-CI) 50 μg/mL ascorbic acid (Sigma, A8960), 10 μg/mL Transferrin (Athens Research and Technology, 16-16-A32001-LEL), 0.1 mM 2-mercaptoethanol (Gibco, 21985023) and 1× Glutagro (Corning, 25-015-CI). .. B-8 medium was generated by adding the following eight supplements to DM, 200 ng/mL LONG R3 human IGF-I (Sigma, 85580 C), 8 ng/mL human basic-FGF (R&D Systems, 4114-TC), 100 ng/mL human-BMP7 (R&D Systems, 354-BP/CF), 10 μM Y27632 (R&D Systems, 1254/50), 2 μM Rosiglitazone (R&D Systems, 5325/50), 1 nM Triiodo-L-thyronine (Sigma, 64245-250MG), 1 μM Dexamethasone (R&D Systems, 1126/100) and 500 μM Isobutylmethylxanthine (Sigma, I5879-5G). ..

    Article Title: MYC CONTROLS HUMAN PLURIPOTENT STEM CELL FATE DECISIONS THROUGH REGULATION OF METABOLIC FLUX
    Article Snippet: .. Definitive endoderm (DE) cells were generated by culturing hESCs or hiPSCs at 50,000 cells/cm 2 on Geltrex, diluted 1:200 with DMEM/F-12, coated plates for 4 days in DM supplemented with 100 ng/mL Activin A and 8 ng/mL human basic-FGF, with 25 ng/mL human Wnt-3a (R&D Systems, 1324-WN-500/CF) added to the media for the first 24 hr only. ..

    Cell Culture:

    Article Title: Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State.
    Article Snippet: DM was composed of DMEM/F-12 w/o glutamine supplemented with 2% Probumin (EMD Milipore, 821005), 1x Antibiotic-Antimyotic (Corning, 30-004-CI), 1x MEM non-essential amino acids (Corning, 25-025-CI), 1x Trace Elements A (Corning, 99-182-CI), 1x Trace Elements B (Corning, 99-175-CI), 1x Trace Elements C (Corning, 99-176-CI) 50 mg/mL Ascorbic Acid (SigmaAldrich, A8960), 10 mg/mL Transferrin (Athens Research and Technology, 16-16-A32001-LEL), 0.1 mM 2-mercaptoethanol (GIBCO,21985023) and 1 x glutagro (Corning, 25-015-CI). .. The hPSC complete maintenance medium (CMM) was composed of DM supplemented with 8 ng/mL human basic-FGF (R&D Systems, 4114-TC), 200 ng/mL LONG R3 human IGF-I (Sigma-Aldrich, 85580C), 10 ng/mL Activin A (R&D Systems, 338-AC-01M/CF) and 10 ng/mL human Heregulin-b 1 (Peprotech, AF-100-03). hESCs and hiPSCs were cultured in CMMwith media changes every 24 hr and passaged at 90% confluency using Accutase (Innovative Cell Technologies, AT104). .. NOD/SCIDmice (NOD/ShiLtSz genetic background, Jackson Laboratory, stock no. 001303) were housed in Tecniplast GM500 ventilated cages in a temperature-controlled room with 12 hr light-12 hr dark cycles.



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